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KMID : 0363620100310020158
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Journal of Korean Oriental Medicine
2010 Volume.31 No. 2 p.158 ~ p.166
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Anti-adipogenic Effect of Kaempferol, a Component of Polygonati Rhizoma
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Jang Jae-Sik
Jung Ji-Chun
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Abstract
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Objective: It has been reported that Polygonati rhizoma (Pr) has anti-hyperglycemia, anti-triglycemia, anti-diabetic, and anti-tumor activity. Total extract of Pr was tested to identify anti-adipogenic activity in 3T3-L1 differentiation and molecular mechanism of Pr in 3T3-L1 differentiation.
Methods: Differentiation of 3T3-L1 pre-adipocyte was induced in the presence of Pr extract and kaempferol. The level of lipid accumulation was measured by Oil Red O staining. The expression of genes associated with adipocyte differentiation was measured by RT-PCR.
Results: Extract of Pr and its component kaempferol reduced lipid accumulation in 3T3-L1 during adipogenesis and also reduced mRNA levels of genes associated with adipogenesis, such as adipsin, aP2, LPL, SERBP-1c and PPARγ.
Conclusions: In this study, we showed that the molecular mechanism of Pr and kaempferol activity is related to regulation of PPARγ expression and activation.
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KEYWORD
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Polygonati rhizoma, kaempferol, adipogenesis, 3T3-L1 differentiation, PPARγ, SREPB-1c
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